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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20147 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepC4-RHE
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Backbone manufacturerARIAD Pharmaceuticals
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLyn-linker-FRB (LDR)
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Alt nameFRAP
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SpeciesH. sapiens (human)
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Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lyn11-targeted FRB (LDR) was a gift from Tobias Meyer (Addgene plasmid # 20147 ; http://n2t.net/addgene:20147 ; RRID:Addgene_20147) -
For your References section:
An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways. Inoue T, Heo WD, Grimley JS, Wandless TJ, Meyer T. Nat Methods. 2005 Jun . 2(6):415-8. 10.1038/nmeth763 PubMed 15908919
