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pBA407-Neo-COVGT5-mCherry
(Plasmid #201455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBA407 (Plasmid #85970)
  • Backbone manufacturer
    Weissmann Lab
  • Backbone size w/o insert (bp) 9256
  • Vector type
    Lentiviral, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    COVGT5_mCherry
  • Alt name
    COVGT5
  • Alt name
    COVGT4+COVGT1_mCherry
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    10473

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We used SARS-CoV-2 signature & transcription factor genes to design reporters that model infection response in epithelial cells (Blanko-Melo et al.,2020; Wyler et al.,2020; Lamers et al.,2020). 5' cloning site: BamHI (unknown if destroyed), 3' cloning site: HpaI (unknown if destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA407-Neo-COVGT5-mCherry was a gift from Gaetano Gargiulo (Addgene plasmid # 201455 ; http://n2t.net/addgene:201455 ; RRID:Addgene_201455)

  • For your References section:

    Pharmacological modulators of epithelial immunity uncovered by synthetic genetic tracing of SARS-CoV-2 infection responses. Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, Serresi M, Cicin-Sain L, Gargiulo G. Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. Epub 2023 Jun 21. 10.1126/sciadv.adf4975 PubMed 37343108
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