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pPB-Neo-COVGT2-d2EGFP-mCherry
(Plasmid #201452)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201452 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Mammalian PiggyBac Expression Vector
  • Backbone manufacturer
    Vectorbuilder
  • Backbone size w/o insert (bp) 7890
  • Vector type
    Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    COVGT2_d2EGFP-mCherry
  • Alt name
    COVGT2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8645

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We used SARS-CoV-2 signature & transcription factor genes to design reporters that model infection response in epithelial cells (Blanko-Melo et al.,2020; Wyler et al.,2020; Lamers et al.,2020). 5' cloning site: AccI (unknown if destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-Neo-COVGT2-d2EGFP-mCherry was a gift from Gaetano Gargiulo (Addgene plasmid # 201452 ; http://n2t.net/addgene:201452 ; RRID:Addgene_201452)

  • For your References section:

    Pharmacological modulators of epithelial immunity uncovered by synthetic genetic tracing of SARS-CoV-2 infection responses. Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, Serresi M, Cicin-Sain L, Gargiulo G. Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. Epub 2023 Jun 21. 10.1126/sciadv.adf4975 PubMed 37343108
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