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Addgene

pJSH-B14
(Plasmid #20145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC-B
  • Backbone manufacturer
    Stratagene (now Agilent)
  • Backbone size w/o insert (bp) 3469
  • Vector type
    Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    From Stratagene's "Strataclone" kit, using their "SoloPack Competent Cells" which are endA-, recA-, tonA-, restriction minus, and F-, and resistant to streptomycin.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Artificial sequence
  • Alt name
    HHIC
  • Insert Size (bp)
    111
  • Mutation
    n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (blunt, see vendor) (not destroyed)
  • 3′ cloning site None (blunt, see vendor) (not destroyed)
  • 5′ sequencing primer M13-20
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

May linearize backbone using the single XmnI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJSH-B14 was a gift from Glen Hartman (Addgene plasmid # 20145 ; http://n2t.net/addgene:20145 ; RRID:Addgene_20145)

  • For your References section:

    Exogenous Controls Increase Negative Call Veracity in Multiplexed, Quantitative PCR Assays for Phakopsora pachyrhizi. Haudenshield JS, Hartman GL. Plant Dis. 2011 Mar;95(3):343-352. doi: 10.1094/PDIS-01-10-0023. 10.1094/PDIS-01-10-0023 PubMed 30743502
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