pDEST27-Vinculin
(Plasmid
#20144)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST27
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 8123
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepDEST27-Vinculin
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Alt nameVinculin
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SpeciesM. musculus (mouse)
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Insert Size (bp)3200
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GenBank IDNM_009502
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Entrez GeneVcl (a.k.a. 9430097D22)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST27-Vinculin was a gift from Valeri Vasioukhin (Addgene plasmid # 20144 ; http://n2t.net/addgene:20144 ; RRID:Addgene_20144) -
For your References section:
Alpha-E-catenin binds to dynamitin and regulates dynactin-mediated intracellular traffic. Lien WH, Gelfand VI, Vasioukhin V. J Cell Biol. 2008 Dec 15. 183(6):989-97. 10.1083/jcb.200805041 PubMed 19075109
Map uploaded by the depositor.