GFP-cdc42prenyl
(Plasmid #20142)

• Depositing Lab: Alaa El-Husseini
• Publication: Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: LB medium
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cell division cycle 42 isoform 1
• Alt name: cdc42 WT prenyl
• Species: H. sapiens (human)
• Insert Size (bp): 600
• GenBank ID: NP_001034891
• Entrez Gene: CDC42 (a.k.a. CDC42Hs, G25K, TKS)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: sequence
• 3′ sequencing primer: sequence

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry