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Addgene

pET-28b(+)/ADK C77S
(Plasmid #201409)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201409 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28b(+)
  • Backbone size w/o insert (bp) 5146
  • Total vector size (bp) 5800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Escherichia coli adenylate kinase C77S
  • Alt name
    ADK C77S
  • Species
    Escherichia coli
  • Insert Size (bp)
    654
  • Mutation
    changed cysteine 77 to serine
  • Entrez Gene
    adk (a.k.a. b0474, ECK0468, dnaW, plsA)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthesized by Gene Universal, Inc.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28b(+)/ADK C77S was a gift from Gary Pielak (Addgene plasmid # 201409 ; http://n2t.net/addgene:201409 ; RRID:Addgene_201409)

  • For your References section:

    (1)H, (13)C, (15)N backbone resonance assignment of Escherichia coli adenylate kinase. Brom JA, Samsri S, Petrikis RG, Parnham S, Pielak GJ. Biomol NMR Assign. 2023 Aug 26. doi: 10.1007/s12104-023-10147-1. 10.1007/s12104-023-10147-1 PubMed 37632688
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