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pcDNA3.1/nV5-DEST-beta catenin
(Plasmid #20140)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20140 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/nV5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7136
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pcDNA3.1/nV5-DEST-beta catenin
  • Alt name
    beta catenin
  • Species
    M. musculus (mouse)
  • Mutation
    T3I
  • Entrez Gene
    Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/nV5-DEST-beta catenin was a gift from Valeri Vasioukhin (Addgene plasmid # 20140 ; http://n2t.net/addgene:20140 ; RRID:Addgene_20140)

  • For your References section:

    Alpha-E-catenin binds to dynamitin and regulates dynactin-mediated intracellular traffic. Lien WH, Gelfand VI, Vasioukhin V. J Cell Biol. 2008 Dec 15. 183(6):989-97. 10.1083/jcb.200805041 PubMed 19075109
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