pcDNA3.1/cyto-iAspSnFR
(Plasmid #201394)

• Purpose: CMV overexpression of cyto-iAspSnFR in mammalian cell lines
• Depositing Lab: Kai Johnsson
• Publication: Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(24)00179-X. doi: 10.1016/j.chembiol.2024.05.002.

Backbone

• Vector backbone: pcDNA3.1/Neo
• Backbone size w/o insert (bp): 5361
• Total vector size (bp): 7623
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyto-iAspSnFR
• Species: Synthetic
• Insert Size (bp): 2262
• Mutation: gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W, Q234R
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV_Fwd

Resource Information

• Supplemental Documents:
  • 028032-1.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.05.04.537313 for bioRxiv preprint.