H_NES 47kDa
(Plasmid
#201348)
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PurposeNucleocytoplasmic transport probe
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIV_NES-EGFP-3PrA
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal lab code: IG041/P294
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H_NES 47kDa was a gift from Pere Roca-Cusachs (Addgene plasmid # 201348 ; http://n2t.net/addgene:201348 ; RRID:Addgene_201348) -
For your References section:
Mechanical force application to the nucleus regulates nucleocytoplasmic transport. Andreu I, Granero-Moya I, Chahare NR, Clein K, Molina-Jordan M, Beedle AEM, Elosegui-Artola A, Abenza JF, Rossetti L, Trepat X, Raveh B, Roca-Cusachs P. Nat Cell Biol. 2022 Jun;24(6):896-905. doi: 10.1038/s41556-022-00927-7. Epub 2022 Jun 9. 10.1038/s41556-022-00927-7 PubMed 35681009