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Addgene

pPM47 (UPR-RFP CEN/ARS URA3)
(Plasmid #20132)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20132 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4835
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UPR-RFP
  • Alt name
    4xUPRE-mCherry
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1419

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a 2 base gap between depositor's sequence and Addgene sequence; per depositor this gap is in the promoter region and shouldn't significantly affect the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPM47 (UPR-RFP CEN/ARS URA3) was a gift from Feroz Papa (Addgene plasmid # 20132 ; http://n2t.net/addgene:20132 ; RRID:Addgene_20132)

  • For your References section:

    Real-time redox measurements during endoplasmic reticulum stress reveal interlinked protein folding functions. Merksamer PI, Trusina A, Papa FR. Cell. 2008 Nov 28. 135(5):933-47. 10.1016/j.cell.2008.10.011 PubMed 19026441
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