pWalium Htt19Q-mCherry
(Plasmid
#201248)
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Purposeallows the expression of the insert in Drosophila melanogaster
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepWalium
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuntingtin
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SpeciesH. sapiens (human)
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Entrez GeneHTT (a.k.a. HD, IT15, LOMARS)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Huntingtin fused with mCherry tag
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWalium Htt19Q-mCherry was a gift from Pedro Domingos (Addgene plasmid # 201248 ; http://n2t.net/addgene:201248 ; RRID:Addgene_201248) -
For your References section:
Protein phosphatase 1 regulates huntingtin exon 1 aggregation and toxicity. Branco-Santos J, Herrera F, Pocas GM, Pires-Afonso Y, Giorgini F, Domingos PM, Outeiro TF. Hum Mol Genet. 2017 Oct 1;26(19):3763-3775. doi: 10.1093/hmg/ddx260. 10.1093/hmg/ddx260 PubMed 28934390