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Addgene

pUASt-Xbp1-HA-GFP-3'UTR Spl
(Plasmid #201246)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201246 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8900
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dXbp1
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1888
  • Entrez Gene
    Xbp1 (a.k.a. Dmel_CG9415, 138/3, CG 9415, CG9415, DM18, Dmel\CG9415, Dxbp-1, XBP-1, XBP1, Xbp-1, XbpI, anon-EST:Liang-1.41, clone 1.41, dXbp-1, dmXBP1, l(2)k13803, sXbp1, xbp-1, xbp1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

xbp1 unspliced tagged with HA and Xbp1 truncated form tagged with GFP, both followed by 3'UTR of spliced form

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASt-Xbp1-HA-GFP-3'UTR Spl was a gift from Pedro Domingos (Addgene plasmid # 201246 ; http://n2t.net/addgene:201246 ; RRID:Addgene_201246)

  • For your References section:

    Pumilio protects Xbp1 mRNA from regulated Ire1-dependent decay. Cairrao F, Santos CC, Le Thomas A, Marsters S, Ashkenazi A, Domingos PM. Nat Commun. 2022 Mar 24;13(1):1587. doi: 10.1038/s41467-022-29105-x. 10.1038/s41467-022-29105-x PubMed 35332141
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