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Addgene

SuExp-mPLD3
(Plasmid #201245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SuExp
  • Backbone manufacturer
    Nemazee lab
  • Backbone size w/o insert (bp) 1900
  • Total vector size (bp) 3400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLD3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • Entrez Gene
    Pld3 (a.k.a. Sam-9)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.20.567917 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SuExp-mPLD3 was a gift from David Nemazee (Addgene plasmid # 201245 ; http://n2t.net/addgene:201245 ; RRID:Addgene_201245)

  • For your References section:

    Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4. Yuan M, Peng L, Huang D, Gavin A, Luan F, Tran J, Feng Z, Zhu X, Matteson J, Wilson IA, Nemazee D. Structure. 2024 Jun 6;32(6):766-779.e7. doi: 10.1016/j.str.2024.02.019. Epub 2024 Mar 26. 10.1016/j.str.2024.02.019 PubMed 38537643
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