SuExp-hPLD3
(Plasmid
#201243)
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Purposeexpress full length human PLD3 protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201243 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSuExp
- Backbone size w/o insert (bp) 1900
- Total vector size (bp) 3400
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLD3
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Alt nameAD19; HUK4; HU-K4; SCA46
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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GenBank ID23646
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Entrez GenePLD3 (a.k.a. AD19, HU-K4, HUK4, SCA46)
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.20.567917 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SuExp-hPLD3 was a gift from David Nemazee (Addgene plasmid # 201243 ; http://n2t.net/addgene:201243 ; RRID:Addgene_201243) -
For your References section:
Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4. Yuan M, Peng L, Huang D, Gavin A, Luan F, Tran J, Feng Z, Zhu X, Matteson J, Wilson IA, Nemazee D. Structure. 2024 Jun 6;32(6):766-779.e7. doi: 10.1016/j.str.2024.02.019. Epub 2024 Mar 26. 10.1016/j.str.2024.02.019 PubMed 38537643