35S-VP16-GWY/pAM
(Plasmid
#201234)
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PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAM
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Vector typePlant Expression
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Selectable markersAgrobacterium - carbenicillin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsWhen using in Agrobacterium, this plasmid can only replicate in strains containing the RK2 helper sequence, such as GV3103 pMP90 RK.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name35S-VP16-GWY
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SpeciesSynthetic
- Promoter CaMV 35S
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Encodes VP16 activation domain in C-terminal juxtaposition to a Gateway cloning site with the ORF under the regulatory control of a CaMV 35S promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
35S-VP16-GWY/pAM was a gift from Michael Ayliffe (Addgene plasmid # 201234 ; http://n2t.net/addgene:201234 ; RRID:Addgene_201234) -
For your References section:
A split GAL4 RUBY assay for visual in planta detection of protein-protein interactions. Chen J, Luo M, Hands P, Rolland V, Zhang J, Li Z, Outram M, Dodds P, Ayliffe M. Plant J. 2023 Jun;114(6):1209-1226. doi: 10.1111/tpj.16234. Epub 2023 Apr 26. 10.1111/tpj.16234 PubMed 37323061