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35S-GAL4BD-GWY/pAM
(Plasmid #201233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAM
  • Vector type
    Plant Expression
  • Selectable markers
    Agrobacterium - carbenicillin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    When using in Agrobacterium, this plasmid can only replicate in strains containing the RK2 helper sequence, such as GV3103 pMP90 RK.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    35S-GAL4BD-GWY
  • Species
    Synthetic
  • Promoter CaMV 35S

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes GAL4 DNA binding domain in C-terminal juxtaposition to a Gateway cloning site with the ORF under the regulatory control of a CaMV 35S promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    35S-GAL4BD-GWY/pAM was a gift from Michael Ayliffe (Addgene plasmid # 201233 ; http://n2t.net/addgene:201233 ; RRID:Addgene_201233)

  • For your References section:

    A split GAL4 RUBY assay for visual in planta detection of protein-protein interactions. Chen J, Luo M, Hands P, Rolland V, Zhang J, Li Z, Outram M, Dodds P, Ayliffe M. Plant J. 2023 Jun;114(6):1209-1226. doi: 10.1111/tpj.16234. Epub 2023 Apr 26. 10.1111/tpj.16234 PubMed 37323061
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