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GAL4BD-VP16/pDON207
(Plasmid #201230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDON207
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    When using in Agrobacterium, this plasmid can only replicate in strains containing the RK2 helper sequence, such as GV3103 pMP90 RK.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAL4BD-VP16
  • Species
    Synthetic

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes a chimeric transcription factor ORF consisting of the yeast GAL4 DNA binding domain and HSV VP16 activation domain. No promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GAL4BD-VP16/pDON207 was a gift from Michael Ayliffe (Addgene plasmid # 201230 ; http://n2t.net/addgene:201230 ; RRID:Addgene_201230)

  • For your References section:

    A split GAL4 RUBY assay for visual in planta detection of protein-protein interactions. Chen J, Luo M, Hands P, Rolland V, Zhang J, Li Z, Outram M, Dodds P, Ayliffe M. Plant J. 2023 Jun;114(6):1209-1226. doi: 10.1111/tpj.16234. Epub 2023 Apr 26. 10.1111/tpj.16234 PubMed 37323061
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