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Addgene

PCRII-zSynC
(Plasmid #20123)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20123 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3961
  • Vector type
    in-situ
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zSynC
  • Alt name
    sncb
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    417
  • Entrez Gene
    sncb (a.k.a. MGC66343, zgc:66343)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site TOPO (unknown if destroyed)
  • 3′ cloning site TOPO (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

zSynC in situ probe.
For antisense, cut with BamHI and transcribe with T7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCRII-zSynC was a gift from Aaron Gitler (Addgene plasmid # 20123 ; http://n2t.net/addgene:20123 ; RRID:Addgene_20123)
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