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Addgene

PCRII-zSynB
(Plasmid #20122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3961
  • Vector type
    in-situ
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zSynB
  • Alt name
    sncgb
  • Alt name
    zgc:110133
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    453
  • Entrez Gene
    sncga (a.k.a. MGC123342, im:6900770, zgc:110133, zgc:123342)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site TOPO (unknown if destroyed)
  • 3′ cloning site TOPO (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

zSynB in situ probe.
For antisense, cut with XhoI and transcribe with SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCRII-zSynB was a gift from Aaron Gitler (Addgene plasmid # 20122 ; http://n2t.net/addgene:20122 ; RRID:Addgene_20122)
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