PCRII-zSynB
(Plasmid
#20122)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePCRII-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3961
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Vector typein-situ
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namezSynB
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Alt namesncgb
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Alt namezgc:110133
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)453
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Entrez Genesncga (a.k.a. MGC123342, im:6900770, zgc:110133, zgc:123342)
Cloning Information
- Cloning method TOPO Cloning
- 5′ cloning site TOPO (unknown if destroyed)
- 3′ cloning site TOPO (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
zSynB in situ probe.
For antisense, cut with XhoI and transcribe with SP6
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PCRII-zSynB was a gift from Aaron Gitler (Addgene plasmid # 20122 ; http://n2t.net/addgene:20122 ; RRID:Addgene_20122)