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pMM7
(Plasmid #201213)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201213 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMTL61110
  • Backbone manufacturer
    Sheng et al. 2017
  • Total vector size (bp) 6091
  • Modifications to backbone
    Incorporation of thermostable sfGFP into vector backbone, addition of homologous flanks for targeted deletion of spo0A in the genome of the P. thermoglucosidasius.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 6.25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    If grown in P. thermoglucosidasius: 12.5 mg/L kanamycin, 52 °C
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sfGFP
  • Alt name
    Superfolder Green Fluorescent Protein
  • Insert Size (bp)
    717
  • Mutation
    F11V, T13P, N39D, A179A

Gene/Insert 2

  • Gene/Insert name
    Spo0A right flank
  • Alt name
    P. thermoglucosidasius
  • Insert Size (bp)
    750

Gene/Insert 3

  • Gene/Insert name
    Spo0A left flank
  • Alt name
    P. thermoglucosidasius
  • Insert Size (bp)
    750

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM7 was a gift from Alex Nielsen (Addgene plasmid # 201213 ; http://n2t.net/addgene:201213 ; RRID:Addgene_201213)

  • For your References section:

    An improved integrative GFP-based vector for genetic engineering of Parageobacillus thermoglucosidasius facilitates the identification of a key sporulation regulator. Millgaard M, Bidart GN, Pogrebnyakov I, Nielsen AT, Welner DH. AMB Express. 2023 May 8;13(1):44. doi: 10.1186/s13568-023-01544-9. 10.1186/s13568-023-01544-9 PubMed 37154828
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