SpyCatcher-SWG
(Plasmid #201194)

• Purpose: Expresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteria
• Depositing Lab: Vesa Hytönen
• Publication: Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52. doi: 10.1039/d4na00483c.

Backbone

• Vector backbone: pET-11b
• Backbone size w/o insert (bp): 5676
• Total vector size (bp): 5961
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyCatcher-SWG
• Alt name: SWG
• Species: H. sapiens (human), M. musculus (mouse), Synthetic
• Insert Size (bp): 369
• Promoter: T7
• Tag / Fusion Protein:
  • HisTag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BlpI (not destroyed)
• 5′ sequencing primer: gagatctcgatcctctacg
• 3′ sequencing primer: caaaaaacccctcaaga

Resource Information

• Supplemental Documents:
  • SpyCatcher-SWG.gbk
• A portion of this plasmid was derived from a plasmid made by: The gene was syntesized and cloned by Genscript.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original publications:
SpyCatcher:
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. PMID: 22366317; PMCID: PMC3311370.