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M2e-noro-VLP
(Plasmid #201192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOET5.1
  • Backbone manufacturer
    Oxford Expression Technologies
  • Backbone size w/o insert (bp) 4573
  • Total vector size (bp) 6258
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    M2e-noro-VLP
  • Alt name
    Noro-M2e
  • Species
    H. sapiens (human); Human norovirus, Human influenza virus
  • Insert Size (bp)
    1685
  • GenBank ID
    AFV08795
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • Influenza M2e peptide (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer taagtattttactgttttcgtaacagttttgtaataaaaaaacct
  • 3′ sequencing primer agaatctagcgcttaataaatgtactaataacaatgtatcg
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Gene synthesis and subcloning was done as service at Genscript, USA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M2e-noro-VLP was a gift from Vesa Hytönen (Addgene plasmid # 201192 ; http://n2t.net/addgene:201192 ; RRID:Addgene_201192)

  • For your References section:

    SpyTag/SpyCatcher display of influenza M2e peptide on norovirus-like particle provides stronger immunization than direct genetic fusion. Lampinen V, Grohn S, Soppela S, Blazevic V, Hytonen VP, Hankaniemi MM. Front Cell Infect Microbiol. 2023 Jun 22;13:1216364. doi: 10.3389/fcimb.2023.1216364. eCollection 2023. 10.3389/fcimb.2023.1216364 PubMed 37424789
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