pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald
(Plasmid #201187)

• Purpose: Lentiviral vector plasmid expressing human PTPN2 transcript variant 2 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
• Depositing Lab: Bart Nieuwenhuis
• Publication: PTPN2 (unpublished)

Backbone

• Vector backbone: pHRsinUbEm
• Total vector size (bp): 11936
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: PTPN2, transcript variant 2
• Alt name: Protein Tyrosine Phosphatase Non-Receptor Type 2, transcript variant 2
• Alt name: TCELLPTP, transcript variant 2
• Alt name: TCPTP, transcript variant 2
• Species: H. sapiens (human)
• Insert Size (bp): 1161
• Entrez Gene: PTPN2 (a.k.a. PTN2, PTPT, TC-PTP, TC45, TC48, TCELLPTP, TCPTP)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotHI (not destroyed)
• 5′ sequencing primer: -

Gene/Insert 2

• Gene/Insert name: Emerald
• Alt name: EmGFP
• Species: A. victoria
• Insert Size (bp): 717

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pLTR-SFFV-PTPN2 transcript variant 2-WPRE-UbC-Emerald.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry