pcDNA3.1-NINJ1
(Plasmid
#201180)
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PurposeTransient expression of NINJ1 gene in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 201180 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1Hygro(+)
- Backbone size w/o insert (bp) 5597
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNinjurin 1
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Alt nameNINJ1
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SpeciesM. musculus (mouse)
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Insert Size (bp)500
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Entrez GeneNinj1
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7_fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
AE16
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-NINJ1 was a gift from Jonathan Kagan (Addgene plasmid # 201180 ; http://n2t.net/addgene:201180 ; RRID:Addgene_201180) -
For your References section:
Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death. Borges JP, Saetra RSR, Volchuk A, Bugge M, Devant P, Sporsheim B, Kilburn BR, Evavold CL, Kagan JC, Goldenberg NM, Flo TH, Steinberg BE. Elife. 2022 Dec 5;11:e78609. doi: 10.7554/eLife.78609. 10.7554/eLife.78609 PubMed 36468682
