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Addgene

pcDNA3.1-NINJ1
(Plasmid #201180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1Hygro(+)
  • Backbone size w/o insert (bp) 5597
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ninjurin 1
  • Alt name
    NINJ1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    500
  • Entrez Gene
    Ninj1
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7_fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AE16

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-NINJ1 was a gift from Jonathan Kagan (Addgene plasmid # 201180 ; http://n2t.net/addgene:201180 ; RRID:Addgene_201180)

  • For your References section:

    Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death. Borges JP, Saetra RSR, Volchuk A, Bugge M, Devant P, Sporsheim B, Kilburn BR, Evavold CL, Kagan JC, Goldenberg NM, Flo TH, Steinberg BE. Elife. 2022 Dec 5;11:e78609. doi: 10.7554/eLife.78609. 10.7554/eLife.78609 PubMed 36468682
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