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pRetroX-TRE3G_NT-GSDMD_I105N_NoCys
(Plasmid #201177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroX-TRE3G
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gasdermin D N-terminal domain
  • Alt name
    GSDMD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    831
  • Mutation
    aa1-276 only, I105N, C39A, C57A, C77A, C122A, C265A, C192A
  • Entrez Gene
    Gsdmd (a.k.a. 1810036L03Rik, DF5L, Dfna5l, GsdmD-1, Gsdmdc1, M2-4)
  • Promoter TRE3G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LNCX_fwd
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AF03

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroX-TRE3G_NT-GSDMD_I105N_NoCys was a gift from Jonathan Kagan (Addgene plasmid # 201177 ; http://n2t.net/addgene:201177 ; RRID:Addgene_201177)

  • For your References section:

    Gasdermin D pore-forming activity is redox-sensitive. Devant P, Borsic E, Ngwa EM, Xiao H, Chouchani ET, Thiagarajah JR, Hafner-Bratkovic I, Evavold CL, Kagan JC. Cell Rep. 2023 Jan 31;42(1):112008. doi: 10.1016/j.celrep.2023.112008. Epub 2023 Jan 19. 10.1016/j.celrep.2023.112008 PubMed 36662620
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