pRetroX-TRE3G_NT-GSDMD_I105N_
(Plasmid #201174)

• Purpose: Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction
• Depositing Lab: Jonathan Kagan
• Publication: Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi: 10.1016/j.celrep.2023.112008. Epub 2023 Jan 19.

Backbone

• Vector backbone: pRetroX-TRE3G
• Backbone size w/o insert (bp): 6600
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gasdermin D N-terminal domain
• Alt name: GSDMD
• Species: M. musculus (mouse)
• Insert Size (bp): 831
• Mutation: aa1-276 only, I105N
• Entrez Gene: Gsdmd (a.k.a. 1810036L03Rik, DF5L, Dfna5l, GsdmD-1, Gsdmdc1, M2-4)
• Promoter: TRE3G

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LNCX_fwd
• 3′ sequencing primer: MSCV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AG10