pRDA_887
(Plasmid #201164)

• Purpose: Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS
• Depositing Lab: John Doench
• Publication: Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi: 10.1016/j.xgen.2023.100387. eCollection 2023 Sep 13.

Backbone

• Vector backbone: pRDA_887
• Total vector size (bp): 9844
• Vector type: Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Puromycin resistance
• gRNA/shRNA sequence: BsmBI Cloning cassette
• Promoter: EFS

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.