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Addgene

pRDA_816
(Plasmid #201157)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRDA_816
  • Total vector size (bp) 12793
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5x-ALFA tag-dEnAsCas12a-VP64
  • Alt name
    5x-tag-dCas12a-VP64
  • Species
    Enhanced Acidaminococcus
  • Insert Size (bp)
    4098
  • Mutation
    D908A
  • Promoter EFS

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRDA_816 was a gift from John Doench (Addgene plasmid # 201157 ; http://n2t.net/addgene:201157 ; RRID:Addgene_201157)

  • For your References section:

    Optimization of Cas12a for multiplexed genome-scale transcriptional activation. Griffith AL, Zheng F, McGee AV, Miller NW, Szegletes ZM, Reint G, Gademann F, Nwolah I, Hegde M, Liu YV, Goodale A, Doench JG. Cell Genom. 2023 Sep 1;3(9):100387. doi: 10.1016/j.xgen.2023.100387. eCollection 2023 Sep 13. 10.1016/j.xgen.2023.100387 PubMed 37719144
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