pAAV-ef1a-DIO-eGCaMP(2+)
(Plasmid
#201150)
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PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(2+)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV_EF1a_DIO
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Vector typeMammalian Expression, Mouse Targeting, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeGCaMP_2+
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.04.13.536801v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-ef1a-DIO-eGCaMP(2+) was a gift from Andre Berndt (Addgene plasmid # 201150 ; http://n2t.net/addgene:201150 ; RRID:Addgene_201150) -
For your References section:
Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium Indicators. Wait SJ, Rappleye M, Lee JD, Goy ME, Smith N, Berndt A. Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi: 10.21203/rs.3.rs-3146778/v1. Preprint. 10.21203/rs.3.rs-3146778/v1 PubMed 37609342