plex_307-EYFP-ccdB-g418
(Plasmid
#201128)
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Purpose(Empty Backbone) pLEX_307 plasmid with N-terminal EYFP tag and g418 marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneplex_307
- Backbone size (bp) 10995
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Vector typeMammalian Expression
- Promoter EF-1α
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- EYFP (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
K207A mutation in EYFP will not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plex_307-EYFP-ccdB-g418 was a gift from Alejandro Chavez (Addgene plasmid # 201128 ; http://n2t.net/addgene:201128 ; RRID:Addgene_201128)