pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}
(Plasmid
#201120)
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PurposeRFP expression in cells under EF-1α promoter; Histone-bound, nuclear
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTurboRFP; H2B-RFP
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SpeciesM. musculus (mouse); Entacmaea quadricolor
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MutationNo
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid generated by VectorBuilder, ID: VB181022-1108dhs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP} was a gift from Ori Bar-Nur (Addgene plasmid # 201120 ; http://n2t.net/addgene:201120 ; RRID:Addgene_201120) -
For your References section:
Exclusive generation of rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells. Zvick J, Tarnowska-Sengul M, Ghosh A, Bundschuh N, Gjonlleshaj P, Hinte LC, Trautmann CL, Noe F, Qabrati X, Domenig SA, Kim I, Hennek T, von Meyenn F, Bar-Nur O. Stem Cell Reports. 2022 Sep 13;17(9):1942-1958. doi: 10.1016/j.stemcr.2022.07.005. Epub 2022 Aug 4. 10.1016/j.stemcr.2022.07.005 PubMed 35931077