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Addgene

pCB11
(Plasmid #201111)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201111 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProTet.E333
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2000
  • Total vector size (bp) 5145
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CcaR
  • Species
    Synechocystis sp. 6803
  • Insert Size (bp)
    704
  • Promoter J23100

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer AAATAGGCGTATCACGAGGC
  • 3′ sequencing primer GAGCGACACGAATTATGCAG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tetA(C)
  • Species
    Escherichia coli
  • Insert Size (bp)
    1191
  • Mutation
    C-to-T transition at pos. no. 1049 of tetA(C) leading to T350I muation in TetA(C)
  • GenBank ID
    AAB59735.1
  • Promoter PcpcG2-172

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGAAGGGGTGAACAGGTCTGG
  • 3′ sequencing primer TCCAGTTCCACCAGAATAGG
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    sfGFP
  • Alt name
    Superfolder GFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter PcpcG2-172

Cloning Information for Gene/Insert 3

  • Cloning method Unknown
  • 5′ sequencing primer ctataccttgtctgcctccc
  • 3′ sequencing primer GCAGGTCGACTCTAGAGGAT
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    tetA insert from Hans-Martin Fischer (Addgene #74110). Plasmid backbone and CcaR and sfGFP inserts are from Jeffrey Tabor (Addgene #63176).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.10.28.514305 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB11 was a gift from Mary Dunlop (Addgene plasmid # 201111 ; http://n2t.net/addgene:201111 ; RRID:Addgene_201111)

  • For your References section:

    Deep model predictive control of gene expression in thousands of single cells. Lugagne JB, Blassick CM, Dunlop MJ. Nat Commun. 2024 Mar 8;15(1):2148. doi: 10.1038/s41467-024-46361-1. 10.1038/s41467-024-46361-1 PubMed 38459057
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