pTrc-CysGA-Im7 WT
(Plasmid
#201081)
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PurposeIm7 WT inserted into CysGA at G364 via GS linker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTrc-99a
- Backbone size w/o insert (bp) 3904
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCysGA-Im7
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SpeciesE. coli
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Insert Size (bp)1077
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTrc-CysGA-Im7 WT was a gift from Shu Quan (Addgene plasmid # 201081 ; http://n2t.net/addgene:201081 ; RRID:Addgene_201081) -
For your References section:
An enzyme-based biosensor for monitoring and engineering protein stability in vivo. Ren C, Wen X, Mencius J, Quan S. Proc Natl Acad Sci U S A. 2021 Mar 30;118(13):e2101618118. doi: 10.1073/pnas.2101618118. 10.1073/pnas.2101618118 PubMed 33753520