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pME map2k2a_s219D
(Plasmid #201058)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201058 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 4762
  • Total vector size (bp) 5956
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAP2k2a
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1194
  • Mutation
    Mutation S219D, constitutively active
  • GenBank ID
    NM_001037391
  • Entrez Gene
    map2k2a (a.k.a. fa56c06, map2k2, mek2, wu:fa56c06, zgc:123171)
  • Promoter none

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME map2k2a_s219D was a gift from Sarah Childs (Addgene plasmid # 201058 ; http://n2t.net/addgene:201058 ; RRID:Addgene_201058)

  • For your References section:

    rasa1-related arteriovenous malformation is driven by aberrant venous signalling. Greysson-Wong J, Rode R, Ryu JR, Chan JL, Davari P, Rinker KD, Childs SJ. Development. 2023 Sep 15;150(18):dev201820. doi: 10.1242/dev.201820. Epub 2023 Sep 14. 10.1242/dev.201820 PubMed 37708300
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