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pSMT3_SRSF1
(Plasmid #201056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSMT3
  • Backbone size w/o insert (bp) 744
  • Total vector size (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SRSF1
  • Alt name
    ASF1/SF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    744
  • Mutation
    none
  • Entrez Gene
    SRSF1 (a.k.a. ASF, NEDFBA, SF2, SF2p33, SFRS1, SRp30a)
  • Tag / Fusion Protein
    • sumo (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMT3_SRSF1 was a gift from Jun Zhang (Addgene plasmid # 201056 ; http://n2t.net/addgene:201056 ; RRID:Addgene_201056)

  • For your References section:

    Peptides that Mimic RS repeats modulate phase separation of SRSF1, revealing a reliance on combined stacking and electrostatic interactions. Fargason T, De Silva NIU, King E, Zhang Z, Paul T, Shariq J, Zaharias S, Zhang J. Elife. 2023 Mar 2;12:e84412. doi: 10.7554/eLife.84412. 10.7554/eLife.84412 PubMed 36862748
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