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Addgene

HE_50
(Bacterial strain #201049)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 201049 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    n/a

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    HE_50
  • Growth instructions
    Growth temperature: recommended 42 °C (37-45 °C). Growth with streptomycin is not required, the parental strain DH10B and this strain HE_50 just happen to be resistant to streptomycin. Grow on LB or 2xYT media as DH10B-derived strains have multiple auxotrophies and this strain obtained more auxotrophies during its evolution in 2xYT media. Please see accompanying paper for more information on the growth temperature range of the strain.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    n/a

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This strain has 572 genetic changes compared to the DH10B reference genome in Genbank (CP000948.1). Please see https://www.ncbi.nlm.nih.gov/nuccore/CP110017 and the accompanying paper for more details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HE_50 was a gift from Francis Nano (Addgene plasmid # 201049)
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