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Addgene

BM28 ΔlysU
(Bacterial strain #201048)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 201048 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    the pOF39 plasmid backbone is pBR325

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BM28 ΔlysU
  • Growth instructions
    Growth temperature: recommended 42-45 °C. Growth with kanamycin is not required as this marker is inserted in the chromosome. Grow on LB media as this strain was evolved in LB media. Grow at ≥42 °C to avoid reversion of thermotolerant phenotype. Please see accompanying papers for more information on the growth temperature range of the strain.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    n/a

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the BM28 ΔlysU genome sequence (https://www.ncbi.nlm.nih.gov/nuccore/CP102379) and the accompanying papers for more details.

Additional publication:
Rudolph B, Gebendorfer KM, Buchner J, Winter J. Evolution of Escherichia coli for growth at high temperatures. J Biol Chem. 2010 Jun 18;285(25):19029-34. doi: 10.1074/jbc.M110.103374. Epub 2010 Apr 20. PMID: 20406805; PMCID: PMC2885180.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BM28 ΔlysU was a gift from Jeannette Winter (Addgene plasmid # 201048)

  • For your References section:

    Whole-genome sequencing analysis of two heat-evolved Escherichia coli strains. McGuire BE, Nano FE. BMC Genomics. 2023 Mar 27;24(1):154. doi: 10.1186/s12864-023-09266-9. 10.1186/s12864-023-09266-9 PubMed 36973666
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