BM28
(Bacterial strain #201047)

• Purpose: MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures. Also carries a pBR325 plasmid carrying groESL called pOF39.
• Depositing Lab: Jeannette Winter
• Publication: McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi: 10.1186/s12864-023-09266-9.

Backbone

• Vector backbone: the pOF39 plasmid backbone is pBR325

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BM28
• Growth instructions: Growth temperature: recommended 42-45 °C. Growth with kanamycin is not required as this marker is inserted in the chromosome. Growth with carbenicillin is recommended to maintain the pBR325 groESL plasmid called pOF39. Grow on LB media as this strain was evolved in LB media. Grow at ≥42 °C to avoid reversion of thermotolerant phenotype. Please see accompanying papers for more information on the growth temperature range of the strain. Copy number: low to medium, likely ~10-20.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: the groESL region region from E. coli is the insert in the pBR325 plasmid pOF39

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the BM28 genome sequence (https://www.ncbi.nlm.nih.gov/nuccore/CP102380) and the accompanying papers for more details.

Additional publications:
Rudolph B, Gebendorfer KM, Buchner J, Winter J. Evolution of Escherichia coli for growth at high temperatures. J Biol Chem. 2010 Jun 18;285(25):19029-34. doi: 10.1074/jbc.M110.103374. Epub 2010 Apr 20. PMID: 20406805; PMCID: PMC2885180.

And for the pOF39 plasmid: Fayet O, Louarn JM, Georgopoulos C. Suppression of the Escherichia coli dnaA46 mutation by amplification of the groES and groEL genes. Mol Gen Genet. 1986 Mar;202(3):435-45. doi: 10.1007/BF00333274. PMID: 3012269.