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PurposeExpresses somatically enriched ASAP4e in neurons, can be used to package AAV
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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How this works
- Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
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- Receive your prep in 6–9 weeks after the MTA is approved by your organization.
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Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameASAP4e-Kv
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-hSyn-ASAP4e-Kv-WPRE was a gift from Michael Lin (Addgene plasmid # 201031 ; http://n2t.net/addgene:201031 ; RRID:Addgene_201031) -
For your References section:
A positively tuned voltage indicator for extended electrical recordings in the brain. Evans SW, Shi DQ, Chavarha M, Plitt MH, Taxidis J, Madruga B, Fan JL, Hwang FJ, van Keulen SC, Suomivuori CM, Pang MM, Su S, Lee S, Hao YA, Zhang G, Jiang D, Pradhan L, Roth RH, Liu Y, Dorian CC, Reese AL, Negrean A, Losonczy A, Makinson CD, Wang S, Clandinin TR, Dror RO, Ding JB, Ji N, Golshani P, Giocomo LM, Bi GQ, Lin MZ. Nat Methods. 2023 Jul;20(7):1104-1113. doi: 10.1038/s41592-023-01913-z. Epub 2023 Jul 6. 10.1038/s41592-023-01913-z PubMed 37429962