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Addgene

pcDNA 3.1-nsp7-FLAG
(Plasmid #201027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 5708
  • Modifications to backbone
    insertion of nsp7-FLAG
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    non-structural protein 7
  • Alt name
    nsp7
  • Species
    Synthetic; Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    327
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • 8xHis (C terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1-nsp7-FLAG was a gift from Sharon Rozovsky (Addgene plasmid # 201027 ; http://n2t.net/addgene:201027 ; RRID:Addgene_201027)

  • For your References section:

    Selenoprotein S Interacts with the Replication and Transcription Complex of SARS-CoV-2 by Binding nsp7. Ghelichkhani F, Gonzalez FA, Kapitonova MA, Rozovsky S. J Mol Biol. 2023 Apr 15;435(8):168008. doi: 10.1016/j.jmb.2023.168008. Epub 2023 Feb 10. 10.1016/j.jmb.2023.168008 PubMed 36773692
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