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Addgene

pTn7-SCOUT14.Y
(Plasmid #201009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTn7-SCOUT11
  • Backbone manufacturer
    Philip Poole lab
  • Backbone size w/o insert (bp) 5253
  • Total vector size (bp) 5522
  • Vector type
    Bacterial Expression
  • Selectable markers
    Streptomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Spectinomycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EC100D pir+
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sYFP2
  • Alt name
    mVenus NB
  • Species
    Synthetic
  • Insert Size (bp)
    865
  • GenBank ID
    DQ092361.1
  • Promoter J23100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer Golden Gate Assembly
  • 3′ sequencing primer Golden Gate Assembly
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTn7-SCOUT14.Y was a gift from Philip Poole (Addgene plasmid # 201009 ; http://n2t.net/addgene:201009 ; RRID:Addgene_201009)

  • For your References section:

    Stable, fluorescent markers for tracking synthetic communities and assembly dynamics. Jorrin B, Haskett TL, Knights HE, Martyn A, Underwood TJ, Dolliver J, Ledermann R, Poole PS. Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2. 10.1186/s40168-024-01792-2 PubMed 38715147
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