pTn7-SCOUT14.G
(Plasmid #201002)

• Purpose: pLv1-pUC18R6KT-miniTn7-aad9-sfGFP, SpR, StR, ApR
• Depositing Lab: Philip Poole
• Publication: Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2.

Backbone

• Vector backbone: pTn7-SCOUT11
• Backbone manufacturer: Philip Poole lab
• Backbone size w/o insert (bp): 5253
• Total vector size (bp): 5516
• Vector type: Bacterial Expression
• Selectable markers: Streptomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Spectinomycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): EC100D pir+
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: superfolder GFP
• Alt name: sfGFP
• Species: Synthetic
• Insert Size (bp): 859
• GenBank ID: ASL68970.1
• Promoter: J23104

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: Golden Gate Assembly
• 3′ sequencing primer: Golden Gate Assembly

Resource Information

• Supplemental Documents:
  • pTn7-SCOUT14.G

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ec100D pir+ genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell.
This strain is naturally resistant to streptomycin

The plasmid appears to be prone to multimerization. Multimerization often does not impact plasmid function, and the depositor has confirmed that the plasmid performs as expected in their hands, but may reduce transformation efficiencies.