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Addgene

pTn7-SCOUT21
(Plasmid #200995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTn7-SCOUT20
  • Backbone manufacturer
    Philip Poole lab
  • Backbone size (bp) 5442
  • Modifications to backbone
    Clone by Golden Gate a gentamycin resistant cassette in the Esp3I site between FRT sequences within the mini-Tn7 transposon
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Gentamicin, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EC100D pir-116
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTn7-SCOUT21 was a gift from Philip Poole (Addgene plasmid # 200995 ; http://n2t.net/addgene:200995 ; RRID:Addgene_200995)

  • For your References section:

    Stable, fluorescent markers for tracking synthetic communities and assembly dynamics. Jorrin B, Haskett TL, Knights HE, Martyn A, Underwood TJ, Dolliver J, Ledermann R, Poole PS. Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2. 10.1186/s40168-024-01792-2 PubMed 38715147
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