pTn7-SCOUT10
(Plasmid
#200989)
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Purpose(Empty Backbone) pL0V-Lv1-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200989 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18R6KT-mini-Tn7T-Km
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Backbone manufacturerHerbert Schweizer
- Backbone size (bp) 4356
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Modifications to backboneRemove BsaI restriction enzyme located in the ampicillin resistant gene (AmpR). Remove a Esp3I restriction site in the backbone located between AmpR and the R-Tn7. The Kanamycin resistant gene located between FRT sequences was replace for a Esp3I restriction sties to clone antibiotic resistant genes by Golden Gate (GG). The multicloning site was replace by a GG level 1 cloning site (BsaI) containing lacZ
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EC100D pir-116
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTn7-SCOUT10 was a gift from Philip Poole (Addgene plasmid # 200989 ; http://n2t.net/addgene:200989 ; RRID:Addgene_200989) -
For your References section:
Stable, fluorescent markers for tracking synthetic communities and assembly dynamics. Jorrin B, Haskett TL, Knights HE, Martyn A, Underwood TJ, Dolliver J, Ledermann R, Poole PS. Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2. 10.1186/s40168-024-01792-2 PubMed 38715147