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Addgene

pTn7-SCOUT10
(Plasmid #200989)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200989 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC18R6KT-mini-Tn7T-Km
  • Backbone manufacturer
    Herbert Schweizer
  • Backbone size (bp) 4356
  • Modifications to backbone
    Remove BsaI restriction enzyme located in the ampicillin resistant gene (AmpR). Remove a Esp3I restriction site in the backbone located between AmpR and the R-Tn7. The Kanamycin resistant gene located between FRT sequences was replace for a Esp3I restriction sties to clone antibiotic resistant genes by Golden Gate (GG). The multicloning site was replace by a GG level 1 cloning site (BsaI) containing lacZ
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EC100D pir-116
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTn7-SCOUT10 was a gift from Philip Poole (Addgene plasmid # 200989 ; http://n2t.net/addgene:200989 ; RRID:Addgene_200989)
  • For your References section:

    Stable, fluorescent markers for tracking synthetic communities and assembly dynamics. Jorrin B, Haskett TL, Knights HE, Martyn A, Underwood TJ, Dolliver J, Ledermann R, Poole PS. Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2. 10.1186/s40168-024-01792-2 PubMed 38715147