pTn7-SCOUT10
(Plasmid #200989)

• Purpose: (Empty Backbone) pL0V-Lv1-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR
• Depositing Lab: Philip Poole
• Publication: Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2.

Backbone

• Vector backbone: pUC18R6KT-mini-Tn7T-Km
• Backbone manufacturer: Herbert Schweizer
• Backbone size (bp): 4356
• Modifications to backbone: Remove BsaI restriction enzyme located in the ampicillin resistant gene (AmpR). Remove a Esp3I restriction site in the backbone located between AmpR and the R-Tn7. The Kanamycin resistant gene located between FRT sequences was replace for a Esp3I restriction sties to clone antibiotic resistant genes by Golden Gate (GG). The multicloning site was replace by a GG level 1 cloning site (BsaI) containing lacZ
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): EC100D pir-116
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pTn7-SCOUT10.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin