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pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)
(Plasmid #200980)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200980 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5332
  • Total vector size (bp) 6526
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EFNB2
  • Alt name
    EphrinB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1194
  • Mutation
    Soluble Receptor-Binding Domain; D62Q-Q130L-V167L mutation for increased specificity to henipaviral G proteins
  • GenBank ID
    NP_004084.1
  • Entrez Gene
    EFNB2 (a.k.a. EPLG5, HTKL, Htk-L, LERK5, ephrin-B2)
  • Promoter CMV
  • Tag / Fusion Protein
    • IgG1 Fc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.26.538420 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L) was a gift from Erik Procko (Addgene plasmid # 200980 ; http://n2t.net/addgene:200980 ; RRID:Addgene_200980)

  • For your References section:

    Sequence basis for selectivity of ephrin-B2 ligand for Eph receptors and pathogenic henipavirus G glycoproteins. Narayanan KK, Amaya M, Tsang N, Yin R, Jays A, Broder CC, Shukla D, Procko E. J Virol. 2023 Nov 30;97(11):e0062123. doi: 10.1128/jvi.00621-23. Epub 2023 Oct 16. 10.1128/jvi.00621-23 PubMed 37931130
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