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Addgene

pET28a-PAN
(Plasmid #200971)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5216
  • Total vector size (bp) 6658
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PAN
  • Species
    Methanocaldococcus jannaschii
  • Insert Size (bp)
    1442
  • Tags / Fusion Proteins
    • 6xHis-thrombin site (N terminal on insert)
    • 6xHis (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-PAN was a gift from Yifan Cheng (Addgene plasmid # 200971 ; http://n2t.net/addgene:200971 ; RRID:Addgene_200971)

  • For your References section:

    Allosteric coupling between alpha-rings of the 20S proteasome. Yu Z, Yu Y, Wang F, Myasnikov AG, Coffino P, Cheng Y. Nat Commun. 2020 Sep 11;11(1):4580. doi: 10.1038/s41467-020-18415-7. 10.1038/s41467-020-18415-7 PubMed 32917864
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