dCas9-CRY2Clust-FUSN-mut
(Plasmid
#200970)
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Purposeinduction of phase-separated condensate mediated by FUSN-IDR mutant
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 200970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX
- Backbone size w/o insert (bp) 6133
- Total vector size (bp) 14234
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Vector typeMammalian Expression, Bacterial Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFUSNmut
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Alt nameFUSN15YS
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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Mutation15 Tyr-to-Ser substitutions
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dCas9-CRY2Clust-FUSN-mut was a gift from Jian Xu (Addgene plasmid # 200970 ; http://n2t.net/addgene:200970 ; RRID:Addgene_200970) -
For your References section:
Light-activated macromolecular phase separation modulates transcription by reconfiguring chromatin interactions. Kim YJ, Lee M Jr, Lee YT, Jing J, Sanders JT, Botten GA, He L, Lyu J, Zhang Y, Mettlen M, Ly P, Zhou Y, Xu J. Sci Adv. 2023 Mar 31;9(13):eadg1123. doi: 10.1126/sciadv.adg1123. Epub 2023 Mar 31. 10.1126/sciadv.adg1123 PubMed 37000871