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Addgene

pND005
(Plasmid #200951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 200951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Backbone manufacturer
    Unknown
  • Backbone size (bp) 6996
  • Vector type
    Yeast Two-hybrid
  • Promoter GAL4
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Timothy Whitehead Lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pND005 was a gift from Timothy Whitehead (Addgene plasmid # 200951 ; http://n2t.net/addgene:200951 ; RRID:Addgene_200951)

  • For your References section:

    Standardizing cassette-based deep mutagenesis by Golden Gate assembly. Daffern N, Francino-Urdaniz IM, Baumer ZT, Whitehead TA. Biotechnol Bioeng. 2024 Jan;121(1):281-290. doi: 10.1002/bit.28564. Epub 2023 Sep 26. 10.1002/bit.28564 PubMed 37750676
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